primary antibodies for rbp4  (R&D Systems)

Journal: Translational Oncology

Article Title: Retinol-binding protein 4 as a promising serum biomarker for the diagnosis and prognosis of hepatocellular Carcinoma

doi: 10.1016/j.tranon.2024.101979

Figure Lengend Snippet: Screening of HCC DEPs via iTRAQ-MALDI-TOF-MS/MS analysis and functional enrichment analyses of DEGs (A-C) The scatter plot for global serum proteins. The red and green dots indicate up- (fold change > 1.2) and down-regulated (fold change < 0.8) DEPs, respectively, when comparing 116 (HCC) versus 113 (HC), 114(CHB) or versus 115(LF). P < 0.05. HC, healthy controls; CHB, chronic hepatitis B virus infection; LF, liver fibrosis; HCC, hepatocellular carcinoma. (D) Venn diagram illustrating 27 DEPs were identified from HC (113) and HCC (116) comparison, 20 DEPs from CHB (114) and HCC (116) comparison, 22 DEPs from LF (115) and HCC (116) comparison and 10 DEPs were shared. HC, healthy controls; CHB, chronic hepatitis B virus infection; LC, liver cirrhosis; HCC, hepatocellular carcinoma; RBP4, retinol-binding protein 4; SERPINA1, serpin family A member 1.

Article Snippet: Tissue sections were blocked for non-specific binding sites with 10 % goat serum and then incubated with RBP4 primary antibody (1:150, Anti-RBP4 antibody, Abcam, Cambridge, MA, USA) at 4 °C overnight.

Techniques: Tandem Mass Spectroscopy, Functional Assay, Virus, Infection, Comparison, Binding Assay

Journal: Translational Oncology

Article Title: Retinol-binding protein 4 as a promising serum biomarker for the diagnosis and prognosis of hepatocellular Carcinoma

doi: 10.1016/j.tranon.2024.101979

Figure Lengend Snippet: RBP4 expression in HCC serum and tissue samples. (A) Relative expression of RBP4 protein in serum as verified by MRM. a, compared with HC, P < 0.0001; b, compared with CHB, P < 0.001. (B) Representative images (400 ×) of positive RBP4 staining in HCC tissues (right panel) and negative staining in adjacent non-tumorous liver tissues (left panel) by IHC staining. Differential expression of RBP4 between (C) cancerous and adjacent non-tumorous tissues ( P < 0.0001) of HCC patients, and between (D) cancerous tissues of AFP-negative (serum AFP < 25 ng/mL) and AFP-positive (serum AFP = 25–400 ng/mL and AFP >400 ng/mL) HCC patients ( P < 0.01) based on semi-quantification of RBP4 IHC. HC, healthy controls; CHB, chronic hepatitis B virus infection; LC, liver cirrhosis; HCC, hepatocellular carcinoma.

Article Snippet: Tissue sections were blocked for non-specific binding sites with 10 % goat serum and then incubated with RBP4 primary antibody (1:150, Anti-RBP4 antibody, Abcam, Cambridge, MA, USA) at 4 °C overnight.

Techniques: Expressing, Staining, Negative Staining, Immunohistochemistry, Virus, Infection

Journal: Translational Oncology

Article Title: Retinol-binding protein 4 as a promising serum biomarker for the diagnosis and prognosis of hepatocellular Carcinoma

doi: 10.1016/j.tranon.2024.101979

Figure Lengend Snippet: Clinical data of 80 cases of RBP4 for HCC.

Article Snippet: Tissue sections were blocked for non-specific binding sites with 10 % goat serum and then incubated with RBP4 primary antibody (1:150, Anti-RBP4 antibody, Abcam, Cambridge, MA, USA) at 4 °C overnight.

Techniques:

Journal: Translational Oncology

Article Title: Retinol-binding protein 4 as a promising serum biomarker for the diagnosis and prognosis of hepatocellular Carcinoma

doi: 10.1016/j.tranon.2024.101979

Figure Lengend Snippet: HCC patient demographic and clinical characteristics.

Article Snippet: Tissue sections were blocked for non-specific binding sites with 10 % goat serum and then incubated with RBP4 primary antibody (1:150, Anti-RBP4 antibody, Abcam, Cambridge, MA, USA) at 4 °C overnight.

Techniques:

Journal: Translational Oncology

Article Title: Retinol-binding protein 4 as a promising serum biomarker for the diagnosis and prognosis of hepatocellular Carcinoma

doi: 10.1016/j.tranon.2024.101979

Figure Lengend Snippet: Serum concentrations of RBP4 by ELISA and receiver operating characteristic (ROC) curve analysis of diagnostic value (A) Serum levels of RBP4 were measured by ELISA in patients with HCC, CHB and healthy controls. a, compared with HC, P < 0.001; b, compared with CHB, P < 0.001. ROC curves for (B) RBP4 alone and RBP4 combined with AFP for all HCC patients versus HC (a, RBP4, AUC = 0.931, 95 % CI: 0.911∼0.950; b, RBP4+AFP, AUC = 0.954, 95 % CI: 0.939∼0.970,R, reference line), (C) AFP negative HCC versus healthy controls (AUC = 0.923, 95 % CI: 0.897∼0.948), (D) AFP positive HCC versus healthy controls (AUC = 0.942, 95 %CI: 0.921∼0.963), (E) RBP4 alone and RBP4 combined with AFP for all HCC patients versus all controls (a, RBP4, AUC = 0.879, 95 % CI: 0.854∼0.903; b,RBP4+AFP, AUC = 0.919, 95 % CI: 0.898∼0.940), (F)AFP negative HCC versus all controls (AUC = 0.875, 95 % CI: 0.843∼0.906) and (G) AFP positive HCC versus all controls (AUC = 0.884, 95 % CI: 0.855∼0.913). (H) CHB versus all HCC (a, RBP4, AUC = 0.809; 95 % CI: 0.771–0.846; b, RBP4+AFP, AUC = 0.872, 95 % CI: 0.840∼0.903; c, AFP, AUC = 0.689, 95 % CI: 0.643∼0.735) (I) Proportion of positive results for AFP, RBP4 or both in patients with HCC. The cutoff value of RBP4 was determined by area under receiver operating characteristic curve (cutoff = 14.06 μg/mL). AFP positive, AFP > 25 ng/mL; AFP negative, AFP < 25 ng/mL; RBP4 positive, RBP4 < 14.06 μg/mL; RBP4 negative, RBP4 > 14.06 μg/mL.

Article Snippet: Tissue sections were blocked for non-specific binding sites with 10 % goat serum and then incubated with RBP4 primary antibody (1:150, Anti-RBP4 antibody, Abcam, Cambridge, MA, USA) at 4 °C overnight.

Techniques: Enzyme-linked Immunosorbent Assay, Diagnostic Assay

Journal: Translational Oncology

Article Title: Retinol-binding protein 4 as a promising serum biomarker for the diagnosis and prognosis of hepatocellular Carcinoma

doi: 10.1016/j.tranon.2024.101979

Figure Lengend Snippet: Results for measurement of serum RBP4, AFP, or both * in the diagnosis of HCC.

Article Snippet: Tissue sections were blocked for non-specific binding sites with 10 % goat serum and then incubated with RBP4 primary antibody (1:150, Anti-RBP4 antibody, Abcam, Cambridge, MA, USA) at 4 °C overnight.

Techniques:

Journal: Translational Oncology

Article Title: Retinol-binding protein 4 as a promising serum biomarker for the diagnosis and prognosis of hepatocellular Carcinoma

doi: 10.1016/j.tranon.2024.101979

Figure Lengend Snippet: Univariable Logistic and Multivariable Cox regression analysis for prognosis of HCC.

Article Snippet: Tissue sections were blocked for non-specific binding sites with 10 % goat serum and then incubated with RBP4 primary antibody (1:150, Anti-RBP4 antibody, Abcam, Cambridge, MA, USA) at 4 °C overnight.

Techniques:

Journal: Translational Oncology

Article Title: Retinol-binding protein 4 as a promising serum biomarker for the diagnosis and prognosis of hepatocellular Carcinoma

doi: 10.1016/j.tranon.2024.101979

Figure Lengend Snippet: Diagnostic value of RBP4 combined prognostic diagnosis model (A) Nomogram to predict overall survival time of HCC. To use this nomogram, the specific point for each variable of the patient lies on each variable axis. Draw a vertical line upward to determine the point at which each variable accepts; the sum of these points is located on the Total Points axis, and draw a vertical line down to the survival axis to determine the probability of 1- and 5- year overall survival time. The length of the line segment reflected the contribution of the factor to the final event. The sum of all individual scores corresponded to the corresponding 1-year and 5-year survival time. (B) ROC curve for HCC prognostic model (a, RBP4+other, AUC = 0.845, 95 % CI: 0.791∼0.899; b, RBP4+AFP+other, AUC = 0.926, 95 % CI: 0.888∼0.964; c, AFP, AUC = 0.809, 95 % CI: 0.747∼0.871).

Article Snippet: Tissue sections were blocked for non-specific binding sites with 10 % goat serum and then incubated with RBP4 primary antibody (1:150, Anti-RBP4 antibody, Abcam, Cambridge, MA, USA) at 4 °C overnight.

Techniques: Diagnostic Assay

Journal:

Article Title: Retinol-Binding Protein Levels are Increased in Association with Gonadotropin Levels in Healthy Women

doi: 10.1016/j.metabol.2008.11.004

Figure Lengend Snippet: Table 2

Article Snippet: Blots were incubated overnight at 4°C with primary antibody directed against RBP4 (A0040, DAKOCytomation, DAKO USA, Carpinteria, CA) diluted 1:1,000 and for 1 hour at room temperature with horseradish-peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA, diluted 1:2,000).

Techniques: